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NASP Antibody
SKU: orb1256714
Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 15
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2). |
| Target | NASP |
| Molecular Weight | Observed: 85kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Nuclear autoantigenic sperm protein, NASP, NASP
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Western blot analysis of extracts of various cell lines, using NASP antibody (orb1256714) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat stomach using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendicitis using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TOM20 antibody (orb1256783) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
NASP
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
NASP Antibody (orb1256714)
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