Native Human Cathepsin D

SKU: orb511144

Description

Cathepsin D purified from human liver and tested to be negative for HbsAg, anti-HBc, anti-HCV, and anti-HIV-1 and -2. This product is best used to study enzyme kinetics, cleave target substrates, and screen for inhibitors.

Images & Validation

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Application Notes

1. Dissolve camptothecin powder in tissue culture grade DMSO to obtain a 2 mg/mL comptothecin stock concentration., 2. Prepare 50 – 100 µL aliquots of the DMSO solubilized camptothecin and store them frozen at < -20°C. A frozen vial of camptothecin may be re-thawed 2X before discarding. Vials which have been thawed 1X should be marked to indicate this so that they only go through one more freeze thaw before being discarded. 3. Spike cell cultures at a camptothecin concentration range of 2 – 4 µg/mL which yields a cell culture camptothecin concentration of 6 – 12 µM. This camptothecin concentration range works well for inducing Jurkat or HL 60 cell suspensions which usually run in the 1 x 105 – 1 x 106 cell/mL concentration range. At this concentration range successful apoptosis induction has been achieved after a 4 hour (37°C) incubation period. 4. Perform time course studies on your particular cell line to ascertain the optimal camptothecin concentration and exposure time required to achieve good apoptosis induction levels for your experiments. 5. Proceed with your experimental apoptosis induction model system.

Key Properties

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Storage & Handling

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Storage	-80°C
Concentration	25 μg protein lyophilized with phosphate buffer
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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