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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
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Item 1 of 10
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Nephrin antibody (orb1239658) was raised against a peptide corresponding to 15 amino acids near the center of human Nephrin. The immunogen is located within amino acids 1100-1150 of Nephrin. |
| Target | NPHS1 |
| Molecular Weight | Predicted: 125, 137 kDa Observed: 125 kDa |
| Purification | Nephrin antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Nephrin antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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