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Nephrin (Y1210) antibody
SKU: orb1938998
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB - 1:2000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 134742 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−NPHN
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All lanes: Anti-Nephrin (Y1210) antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A-498 whole cell lysate. Lane 3: Caki-1 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 135 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Nephrin (Y1210) antibody western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).
Nephrin (Y1210) antibody western blot analysis in mouse kidney tissue lysates (35 ug/lane). This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).
Nephrin Antibody (Y1210) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
Nephrin (Y1210) antibody (orb1938998)
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