Neurotrophin Antibody Kit

SKU: orb3167533

Description

Neurotrophin antibody kit of isotype controls containing a collection of our popular sheep antibodies raised against BDNF.

Images & Validation

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Tested Applications	IHC, NeA
Dilution Range	WB: 1-10 µg/mL; IHC: 1-10 µg/mL; ELISA: 1-10 µg/mL
Reactivity	Human, Mouse, Rat
Application Notes	Immunohistochemistry: 1-10 µg/mL. BDNF antibody works best on Zamboni's fixed, frozen tissue, and is not recommended for paraffin-embedded tissue. ELISA: 1-10 µg/mL. Western Blotting: 1-10 µg/mL. Western blotting is not recommended for the BDNF antibody. Inhibition of biological activity in vitro/in vivo: Expected ED50 (in vivo) values are in the range of 2-10 µg/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies. Read more...

Key Properties

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Host	Sheep
Clonality	Polyclonal
Isotype	IgG
Immunogen	BDNF: recombinant human BDNF NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE) NT3: recombinant human NT3 NT4/5: recombinant human NT4
Target	Neurotrophins
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Lyophilized from PBS, pH 7.4, without preservatives.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

Neurotrophins

UniProt

P23560, P34130, P20783, P01139

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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