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Featured
KO/KD
Validated
Validated
Description
Research Area
Alzheimer's Disease, Cell Biology, Host Immune Response, Host-Virus Interaction, Inflammation, Inflammatory Mediators, Innate Immunity, NF-kB Signalling Pathway, Neuroscience, Obesity, RELA (p65), Virology
Images & Validation
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Item 1 of 8
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-200, IF=1:100-500, Flow-Cyt=1:50-100 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | B5I3 |
| Immunogen | A synthesized peptide derived from human NF-kB p65 (500-551/551aa) |
| Target | RELA |
| Molecular Weight | 65 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AIF3BL3; CMCU; NFKB3; p65; p65 NF-kappa B; p65 NFkB; NFkB; nos2; TF65_HUMAN; RELA; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; TF65_MOUSE; RELA proto-oncogene, NF-kB subunit; v-rel avian reticuloendotheliosis viral oncogene homolog A
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Human
Rabbit
Recombinant
Unconjugated
50 μl, 100 μl, 25 μl
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Quick Database Links
Gene Symbol
RELA
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Available Sizes
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