NGF Recombinant Rabbit Monoclonal Antibody

SKU: orb704404

Description

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Human, Zebrafish

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	11G1
Immunogen	A synthesized peptide derived from human beta NGF (202-241aa)
Target	NGF
Molecular Weight	13/32 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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beta nerve growth factor; NGF-beta; beta nerve growth factor precursor; beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor beta; Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGF B; NID67; NGF beta; NGF-B; NGF_HUMAN.

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NGF Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Lane 3: Heart (Mouse) Lysate at 40 ug, Lane 4: Heart (Rat) Lysate at 40 ug, Lane 5:Spleen (Rat) Lysate at 40 ug, Lane 6: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-NGF (orb704404) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kD, Observed band size: 34 kD.

Quick Database Links

Gene Symbol

NGF

UniProt

P01138

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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