NKX3-1 Antibody (Center)

SKU: orb1928164

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

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Item 1 of 4

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:8000, IHC-P - 1:50-100, FC - 1:10-50
Reactivity	Human
Predicted Reactivity	Mouse

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD45832
Immunogen	This NKX3-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-145 amino acids from the Central region of human NKX3-1. Antigen Region: 3-1 aa.
Target	NKX3-1 (HGNC:7838)
Molecular Weight	26350 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Homeobox protein Nkx-31, Homeobox protein NK-3 homolog A, NKX3-1, NKX31, NKX3A

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of NKX3-1 Antibody (Center) in MCF-7 cell line lysates (35 ug/lane). NKX3-1 (arrow) was detected using the purified Pab.

NKX3-1 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-NKX3-1 Antibody (Center) at 1:8000 dilution + LNCaP whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with NKX3-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.