NME1 Rabbit Polyclonal Antibody

SKU: orb592639

Description

Rabbit polyclonal antibody to NME1

Images & Validation

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Item 1 of 7

Tested Applications	DOT, IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Equine, Guinea pig, Mouse, Rabbit, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human NME1
Target	NME1
Molecular Weight	17kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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NB, AWD, NBS, GAAD, NDKA, NM23, NDPKA, NDPK-A, NM23-H1

Quality Guarantee

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Anti-NME1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Primary Antibody concentration 5 ug/ml.

Anti-NME1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Primary Antibody concentration 5 ug/ml.

DB Suggested Anti-Nme1 antibody, Titration: 0.5 ug/ml, Positive Control: Recomninant human NME2 protein.

Human kidney

Lanes: 1. 20 ug murine 4T1 primary breast tumor cell lysate, 2. 20 ug human MDA-MB-231 cell lysate, 3. 20 ug murine 4T1 primary breast tumor cell lysate, Primary Antibody Dilution: 1:2000, Secondary Antibody: Goat anti-rabbit-Alexa Fluor 680, Secondary Antibody Dilution: 1:25000, Gene Name: NME1.

Lanes: 1. 20 ug murine 4T1 primary breast tumor cell lysate, 2. 20 ug human MDA-MB-231 cell lysate, 3. 20 ug murine 4T1 primary breast tumor cell lysate, Primary Antibody Dilution: 1:4000, Secondary Antibody: Goat anti-rabbit-Alexa Fluor 680, Secondary Antibody Dilution: 1:25000, Gene Name: NME1.

NME1 antibody - N-terminal region (orb592639) validated by WB using Fetal Heart Lysate at 1 ug/ml.

Quick Database Links

Gene Symbol

NME1

UniProt

P22392

RefSeq (Protein):NP_000260

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000260

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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DOT

Dot Blot

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