NOD1 Antibody (C-term)

SKU: orb1937939

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

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Item 1 of 4

Tested Applications	IHC-P, WB
Dilution Range	WB - 1:2000, IHC-P-Leica - 1:500
Reactivity	Human, Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	This NOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-951 amino acids from the C-terminal region of human NOD1. Antigen Region: 923-951 aa.
Target	NOD1 {ECO:0000303\|PubMed:10329646, ECO:0000312\|HGNC:HGNC:16390}
Molecular Weight	107691 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Nucleotide-binding oligomerization domain-containing protein 1, Caspase recruitment domain-containing protein 4, NOD1, CARD4

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

All lanes: Anti-NOD1 Antibody (C-term) at 1:1000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: NCI-H1299 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: SW480 whole cell lysate. Lane 6: Mouse heart lysate. Lane 7: Mouse spleen lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 108 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NOD1 Antibody (C-term) at 1:2000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: NCI-H1299 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: SW480 whole cell lysate. Lane 6: Mouse heart lysate. Lane 7: Mouse spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 108 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded human spleen tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Quick Database Links

Gene Symbol

NOD1 {ECO:0000303|PubMed:10329646, ECO:0000312|HGNC:HGNC:16390}

UniProt

Q9Y239

RefSeq (Protein):NP_006083.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006083.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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