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NPR3 Antibody

SKU: orb1248804

Description

NPR3 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 52kDa band observed in Human Placenta lysates (calculated MW of 59.8kDa according to NP_000899.1). Recommended concentration: 1-3µg/mL.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of brush borders in select PCT. Recommended concentration: 2-4µg/mL. Paraffin embedded Human Prostate. Recommended concentration: 10µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-HRELREDSIRSHFS
TargetNPR3
Molecular WeightApprox 52 kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

NPR3, natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C), ANPRC, GUCY2B, NPRC, ANP-C, ANPR-C, NPR-C, OTTHUMP00000197048, OTTHUMP00000220980, atrial natriuretic peptide clearance receptor, atrial natriuretic peptide receptor 3, atrial natriuretic peptide receptor type C

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

NPR3 Antibody

orb1248804 (1 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NPR3 Antibody

orb1248804 (2 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

NPR3 Antibody

orb1248804 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

NPR3 Antibody

orb1248804 (10 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

NPR3 Antibody (orb1248804)

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0.1 mg
$ 920.00
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