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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF - 1:10-50, WB - 1:1000 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD62451 |
| Immunogen | This NR2C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-466 amino acids from the C-terminal region of human NR2C2. Antigen Region: 438-466 aa. |
| Target | NR2C2 |
| Molecular Weight | 65414 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4, NR2C2, TAK1, TR4
Quality Guarantee
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Fluorescent confocal image of Hela cell stained with NR2C2 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly.
NR2C2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the NR2C2 antibody detected the NR2C2 protein (arrow).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003289.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
NR2C2 Antibody (C-term) (orb1932400)
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