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KO/KD
Validated
Validated
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | KO/KD Validated, WB |
|---|---|
| Dilution Range | WB - 1:2000 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1,κ |
| Clone No. | B4375EV529X54X02 |
| Immunogen | This antibody is generated from a mouse immunized with a recombinant protein from human. |
| Target | NR3C1 (HGNC:7978) |
| Molecular Weight | 85659 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL
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All lanes: Anti-NR3C1 Antibody at 1:1000 dilution (upper) Lane 1: HeLa Lane 2: HeLa-Knockout Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution. Predicted band size: 85 kDa
All lanes: Anti-NR3C1 at dilution. Lane 1: DU145 whole cell lysate. Lane 2: U-251MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 86 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-NR3C1 at dilution. Lane 1: HeLa whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: CCRF-CEM whole cell lysate. Lane 4: CHO whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 86 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
NR3C1 (HGNC:7978)
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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NR3C1 Antibody (orb1925303)
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