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NRF1 Recombinant Mouse Monoclonal Antibody
SKU: orb1152052
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Description
Research Area
Cell Biology, DNA and RNA, DNA and RNA Research, Epigenetics and Nuclear Signaling, Mitochondrial Biogenesis, Transcription Factors, mitochondrial transcription
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, ICC/IF=1:100-500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG2b/Kappa |
| Immunogen | KLH conjugated synthetic peptide derived from human NRF1 |
| Target | NRF1 |
| Molecular Weight | 55 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−erythroid derived 2; like 1; Locus control region factor 1; Locus control region-factor 1; NF-E2-related factor 1; NF2L1_HUMAN; NFE2 related factor 1; NFE2-related factor 1; NFE2L1; NRF-1; NRF 1; Nuclear factor; Nuclear factor erythroid 2-related factor 1; Nuclear factor erythroid derived 2 like 1; TCF-11; TCF11; Transcription factor 11; Transcription factor HBZ17; Transcription factor LCR F1; Transcription factor LCR-F1.
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HeLa, 2: HEK-293, 3: Jurkat, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 54 kDa, Observed MW: 70 kDa.
Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1152052.
Sample: Lane 1: Human Hela cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human SH-SY5Y cell lysates, Lane 4: Human 293T cell lysates, Lane 5: Human Jurkat cell lysates, Primary: Anti-NRF1 (orb1152052) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 70 kDa.
Quick Database Links
Gene Symbol
NRF1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
NRF1 Recombinant Mouse Monoclonal Antibody (orb1152052)
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