NRSF Rabbit Polyclonal Antibody

SKU: orb13623

Featured

Description

Research Area

DNA and RNA Research, Neurogenesis, Neuroscience, Zinc Finger Protein

Images & Validation

−

Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, IP, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, IP=1:50-200
Reactivity	Human
Predicted Reactivity	Bovine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human NRSF (401-450/1097aa)
Target	REST
Molecular Weight	121 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

RE1-Silencing Transcription Factor; NRSF; Neural-Restrictive Silencer Factor; XBR; Neuron Restrictive Silencer Factor; Repressor Binding To The X2 Box; X2 Box Repressor; REST_HUMAN

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

NRSF was immunoprecipitated from human hela cells lysate with orb13623 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution. Lane 1: human hela cells lysate 10 µg (Input). Lane 2: orb13623 IP in human hela cells lysate. Lane 3: native rabbit IgG IP in human hela cells lysate (negative control). Secondary, All lanes: Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000.

Sample: Hela (Human) Cell Lysate at 30 ug, Hela KO NRSF (Human) Cell Lysate at 30 ug, Primary: Anti-NRSF (orb13623) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kD, Observed band size: 121 kD.

Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-NRSF (orb13623) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kD, Observed band size: 121 kD.

Tissue/Cell: human gastric tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated (orb13623) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

REST

UniProt

Q13127

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

Immunofluorescence

Immunoprecipitation