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OPN-a/b Antibody (N-term)
SKU: orb1938103
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | IF - 1:25, WB - 1:1000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 35423 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BNSP, OPN
Quality Guarantee
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All lanes: Anti-OPN-a/b Antibody (N-term) at 1:1000 dilution. Lane 1: human liver lysates. Lane 2: MDA-MB-453 whole cell lysates. Lane 3: MOLT-4 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-OPN-a/b Antibody (N-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HL-60 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: MOLT-4 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-OPN-a/b Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling OPN-a/b at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
OPN-a/b Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of OPN-a/b Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of OPN-a/b (arrow) using rabbit polyclonal OPN-a/b Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OPN-a/b gene.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
OPN-a/b Antibody (N-term) (orb1938103)
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