Osteocalcin Rabbit Polyclonal Antibody

SKU: orb1266

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Description

Research Area

Osteogenesis

Images & Validation

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Item 1 of 7

Tested Applications	ELISA, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Osteocalcin (21-100/100aa)
Target	BGLAP
Molecular Weight	11 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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OSTCN_HUMAN; BGLAP; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate protein; OC; OCN;

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citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium clei.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei stainin.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human MG63 cell lysates, Lane 3: Human A549 cell lysates, Lane 4: Human U-2 OS cell lysates, Primary: Anti-Osteocalcin (orb1266) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 11 kDa.

Quick Database Links

Gene Symbol

BGLAP

UniProt

P02818

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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IHC (1)
WB (1)

Filter by Species

Rat (1)
Human (1)

Fábio Vieira de Miranda-Filho 1, Stéfany Barbosa 1, Olavo Alcalde Panigali 1, Mirela Caroline Silva 1, Monique Gonçalves da Costa 1, Franciele da Silva Flores 1, Edilson Ervolino 2, Letícia Helena Theodoro 1, Osvaldo Magro-Filho 1, Leonardo Perez Faverani 1 Effect of local and systemic administration of atorvastatin for improving bone healing on critical defects Braz Dent J ., (2024)

PubMed: 39476113

Applications

IHC

Reactivity

Rat

Kai Zheng 1 2, Wenming Li 1, Tianhao Wang 3, Gaoran Ge 1, Wei Zhang 1, Yi Qin 1, Wenhao Li 1, Zebin Wu 1, Zhen Wang 4, Gang Rui 2, Yaozeng Xu 1, Dechun Geng Dopamine D1 Receptor Contributes to Glucocorticoid-Associated Osteonecrosis of Femoral Head Protection Through the ATF3/CHOP Axis to Inhibit Osteoblastic Apoptosis Adv Sci (Weinh), (2025)

PubMed: 40583147

Applications

Reactivity

Human