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Osthole

SKU: orb1304936

Description

Osthole is a natural coumarin derivative with research potential as a histamine H1 receptor antagonist. It has been demonstrated in vitro to promote the proliferation and differentiation of osteoblasts, supporting investigations into bone metabolism and skeletal research.

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

Key Properties

CAS Number484-12-8
MW244.29
Purity99.96%
FormulaC15H16O3
SMILESCOC1=C(C\C=C(/C)C)C2=C(C=CC(=O)O2)C=C1
TargetApoptosis,Parasite,Histamine Receptor,HBV
SolubilityH2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:55 mg/mL (225.14 mM);Ethanol:46 mg/mL (188.3 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (8.19 mM)

Bioactivity

In Vivo
Osthol, when introduced to pre-washed rabbit platelets, inhibits platelet aggregation and ATP release induced by ADP, arachidonic acid, platelet-activating factor (PAF), collagen, ionophore A23187, and thrombin. In human osteoblasts, osthol promotes cell differentiation through the bone morphogenetic protein-2 / p38 and extracellular signal-regulated kinase 1/2 pathways. Additionally, in cell cultures, osthol suppresses the secretion of the hepatitis B virus by enhancing the glycosylation of HBsAg.
In Vitro
The LD50 values of Osthole in various animals have been determined as follows: in rats, it exceeds 125 mg/kg when administered intravenously (i.v.); in rabbits, it is over 35 mg/kg (i.v.); and in dogs, it surpasses 150 mg/kg (i.v.).
Cell Research
Osthole is dissolved in 96% ethyl alcohol and then sterilized through a 0.22 μg/mL filter and stored at 4°C as stock solutions for later dilution. Peripheral blood samples are collected from participants between 7.00 and 9.00 a.m. on the first study day and these are concentrated in grouping tubes with K3EDTA. Fresh PBMCs are then prepared. Isolated cells are seeded on 24-well plates at 1×106 per well with RPMI-1640 and supplemented with 1% heat inactivated human AB serum, 1% gentamicin and 0.25% PHA. Active reagents are added to each well after 24 h and pure medium formed the control for each substance. Cells are then harvested after a further three days.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Apoptosis, Hepatitis B virus, HBV, Inhibitor, HistamineReceptor, Histamine Receptor, H1 receptor, NSC 31868, inhibit, NSC31868, NSC-31868, Parasite, Ostol, Osthol, Osthole

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Key Properties

No computed properties available.

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Osthole (orb1304936)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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200 mg
$ 70.00
1 ml x 10 mM (in DMSO)
$ 80.00
500 mg
$ 100.00
1 g
$ 140.00
2 g
$ 190.00
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