Oxidopamine hydrochloride

SKU: orb1219758

Description

Oxidopamine hydrochloride is an neurotransmitter dopamine antagonist.

Images & Validation

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Key Properties

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CAS Number	28094-15-7
MW	205.64
Purity	>98% (HPLC)
Formula	C₈H₁₂ClNO₃
SMILES	Cl.NCCc1cc(O)c(O)cc1O
Target	Dopamine Receptor
Solubility	DMSO:32 mg/mL (155.61 mM)

Bioactivity

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In Vitro

Cell Viability Assay Cell line: Neuro-2a cells and SH-SY5Y cells. Concentration: 0-500 μM. Incubation time: 24 or 48 h. Result: Induced neurotoxicity, caused cytotoxicity in both Neuro-2a cells and SH-SY5Y cells in a concentration dependent manner. EC50 = 111 μM for 24 h incubation and 109 μM for 48 h incubation in the Neuro-2a cells; EC50 = 118 μM for 24 h incubation and 107 μM for 48 h incubation in the SH-SY5Y cells. RT-PCR Cell line: Neuro-2a cells and SH-SY5Y cells. Concentration: 75 or 150 μM. Incubation time: 0, 6 or 24 h. Result: Quickly and robustly induced COX-2 in a time-dependent manner. Induced COX-2 activation characterized by expression induction and nuclear translocation. Substantially increased PGE2 in the culture medium by nearly 5-fold in Neuro-2a cells (at 75 μM) and 3-fold in SH-SY5Y cells (at 150 μM). Significantly upregulated the pro-inflammatory cytokine interleukin-1β (IL-1β) within Neuro-2a cells and SH-SY5Y cells. Apoptosis Analysis Cell line: PC12 cells. Concentration: 0, 25, 50, 75, and 150 μM. Incubation time: 0, 2, 4, 6, 12, and 20 h. Result: Induced apoptosis of PC12 cells. Increased the activities of caspase-3, -8 and -9 in PC12 cells in a time- and concentration-dependent manner. Increased these caspase activities at 2-4 h and reached a maximum at 12 h. Decreased cells with high mitochondrial membrane potential (JC-1 aggregate) in a time- and concentration-dependent manner. Western blot analysis. Cell line: PC12 cells. Concentration: 75 μM. Incubation time: 0, 3, 5, 6, 8, 10, and 12 h. Result: Increased the level of p-p38 in a time-dependent manner.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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6-Hydroxydopamine hydrochloride,6-OHDA hydrochloride

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Gene Symbol

Dopamine Receptor

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