P21 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499284

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Description

Research Area

CIP/KIP Family, CIP/KIP family, Cell Cycle, Coactivators and Corepressors, DNA / RNA Binding, DNA and RNA Research, Epigenetics and Nuclear Signaling, Zinc Finger Protein

Images & Validation

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Item 1 of 4

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, ICC/IF=1:50-200, Flow-Cyt=1:50-100
Reactivity	Human

Related Conjugates & Formulations

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BF488

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	R5H7
Immunogen	A synthesized peptide derived from human CDKN1A (50-90aa)
Target	CDKN1A
Molecular Weight	18 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CDN1A_HUMAN; Cyclin-dependent kinase inhibitor 1; CDKN1A; CAP20; CDKN1; CIP1; MDA6; MDA-6; PIC1; SDI1; WAF1; p21CIP1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6(MDA-6);

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P21 Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF7, 2: U87-MG, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 18 kDa, Observed MW: 18 kDa.

Sample: Lane 1: Human HUVEC cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human U87MG cell lysates, Primary: Anti-p21 (orb1499284) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kDa, Observed band size: 18 kDa.

Tissue: Human cervical carcinoma, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499284.

Tissue: Human thyroid carcinoma, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499284.

Quick Database Links

Gene Symbol

CDKN1A

UniProt

P38936

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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