Recombinant p40 Antibody / deltaNp63

SKU: orb699694

Description

p63 consists of two major isoforms-TAp63 and delta-Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation-competent TA domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, DNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive delta-N domain, which antagonizes the activity of TAp63 and p53. P40/3980R recognizes exclusively delta-Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.

Research Area

Apoptotic, Cancer, Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

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Tested Applications	FACS, IF, IHC-P
Dilution Range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant p40 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rTP40/3690
Immunogen	A synthetic peptide from the N-terminal of human p40 protein was used as the immunogen for the recombinant p40 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human kidney with recombinant p40 antibody (clone rTP40/3690). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skin with recombinant p40 antibody (clone rTP40/3690). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant p40 antibody (clone rTP40/3690); Red=isotype control, Blue= recombinant p40 antibody.

Quick Database Links

UniProt

Q9H3D4

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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