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p53 (phospho Ser392) rabbit pAb Antibody
SKU: orb770300
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Reactivity | Human, Monkey |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser392. AA range:344-393 |
| Molecular Weight | 53 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-TP53 antibody, anti-P53 antibody, anti-Cellular tumor antigen p53 antibody, anti-Antigen NY-CO-13 antibody, anti-Phosphoprotein p53 antibody, anti-Tumor suppressor p53 antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p53 (Phospho-Ser392) Antibody.
Immunofluorescence analysis of HeLa cells, using p53 (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using p53 (Phospho-Ser392) Antibody. The lane on the right is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
p53 (phospho Ser392) rabbit pAb Antibody (orb770300)
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