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p70 S6 kinase α (phospho Ser371) rabbit pAb Antibody
SKU: orb769965
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Description
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| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Ser371. AA range:337-386 |
| Molecular Weight | 60 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-RPS6KB1 antibody, anti-STK14A antibody, anti-Ribosomal protein S6 kinase beta-1 antibody, anti-S6K-beta-1 antibody, anti-S6K1 antibody, anti-70 kDa ribosomal protein S6 kinase 1 antibody, anti-P70S6K1 antibody, anti-p70-S6K 1 antibody, anti-Ribosomal protein S6 kinase I antibody, anti-Serine/threonine-protein kinase 14A antibody, anti-p70 antibody
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Western Blot analysis of COLO cells using Phospho-p70 S6 kinase α (S371) Polyclonal Antibody diluted at 1:500.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p70 S6 Kinase (Phospho-Ser371) Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Ser371) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using p70 S6 Kinase (Phospho-Ser371) Antibody. The lane on the right is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
p70 S6 kinase α (phospho Ser371) rabbit pAb Antibody (orb769965)
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