RPS6KA1 Antibody

SKU: orb345670

Description

p90 RSK1 (phospho-S732) antibody

Images & Validation

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Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:5000-1:50,000, WB: 1:1000-1:3000
Reactivity	Human
Application Notes	This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 pS732 protein is expected in the appropriate cell lysate or extract.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Target	RPS6KA1
Purity	This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein phosphorylated at S732. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Partially cross reactivity may occur against opossum and chicken based on > 90% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.51 mg/mL
Expiration Date	12 months from date of receipt.
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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rabbit anti-p90 RSK1 pS732 antibody, rabbit anti-RSK1 pS732 antibody, Ribosomal S6 Kinase 1, RSK-1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, p90-RSK 1, p90S6K, MAPKAP kinase 1a, MAPKAPK-1a, Ribosomal S6 kinase 1, RSK 1, RPS6KA1, MAPKAPK1A

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western Blot of Rabbit anti-p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysates. Lane 2: EGF stimulated HEK293T cell lysates. Load: 35 µg per lane. Primary antibody: p90 RSK1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: peroxidase conjugated secondary antibody and ECL. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 90 kDa, ~90 kDa for p90 RSK1. Other band(s): none.

Western blot using Biorbyt's affinity purified anti-p90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL.

Western blot using Biorbyt's affinity purified anti-p90 RSK1 pS732 antibody. Lane 1-2: HEK293T (U) untreated or (T) treated with EGF. Lane 3-4: HEK293T (U) untreated or (T) treated with EGF. Lane 5-6: HEK293T (U) untreated or (T) treated with EGF. Load: 15 µg per lane. Actin used as a loading control. Blocking: 5% milk. Primary Antibody: Anti-RSK1-pS732 1:1000, 1:2000, or 1:3000 O/N. Secondary Antibody: Goat Anti-Rabbit IgG 1:5000 for 2 hours. Predicted Size: ~90 kDa in size corresponding to phosphorylated p90 RSK1 in EGF stimulated.

Quick Database Links

Gene Symbol

RPS6KA1

UniProt

Q15418

RefSeq (Protein):NP_001006666.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001006666.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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