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Pan Cytokeratin Antibody Cocktail (Acidic + Basic)
SKU: orb1151900
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 1
| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution Range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | SPM115 + SPM116 |
| Immunogen | Human epidermal keratin was used as the immunogen for the Pan Cytokeratin antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC staining of FFPE human breast carcinoma tissue with Pan Cytokeratin antibody cocktail (clone SPM115 + SPM116). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
Pan Cytokeratin Antibody Cocktail (Acidic + Basic) (orb1151900)
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