PAX5 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499314

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Description

Research Area

Developmental Biology, Neurogenesis, Neuroscience, PAX

Images & Validation

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Item 1 of 3

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P
Dilution Range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100, ICC/IF=1:50-200
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	R6F9
Immunogen	A synthesized peptide derived from human PAX5 (250-300aa)
Target	PAX5
Molecular Weight	42 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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B cell lineage specific activator; B cell lineage specific activator protein; B cell specific activator protein; B cell specific transcription factor; B-cell-specific transcription factor; BSAP; EBB-1; KLP; Paired box 5; Paired box gene 5(B cell lineage specific activator protein); Paired box gene 5(B cell lineage specific activator); Paired box gene 5; Paired box homeotic gene 5; Paired box protein Pax 5; Paired box protein Pax-5; Paired domain gene 5; PAX 5; PAX5_HUMAN; Transcription factor PAX 5.

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PAX5 Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1. Jurkat (Negative control), 2: Ramos, 3: Raji, 4: Daudi, 5: Mouse spleen, 6: Rat, spleen, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 42 kDa, Observed MW: 45 kDa.

Tissue: Human appendix, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high, pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal, for orb1499314.

Tissue: Human kidney (Negative tissue), Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: No staining on nucleus.

Quick Database Links

Gene Symbol

PAX5

UniProt

Q02548

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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