PAX6 Rabbit Polyclonal Antibody

SKU: orb1294411

Description

Rabbit polyclonal antibody to PAX6

Images & Validation

−

Item 1 of 4

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500, Immunofluorescence 1:200–1:300, Immunohistochemistry (Paraffin) 1:100
Reactivity	Human, Mouse, Rat

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the center region.
Target	PAX6
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Anti-PAX6 antibody - 1/500 dilution Lysates at 60 µg per lane. This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294411 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294411 anti-PAX6 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse testis labeling PAX6 with orb1294411 at 1/400.

Quick Database Links

Gene Symbol

PAX6

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Immunofluorescence

View Protocol

Find More Protocols