PAX8 Rabbit Polyclonal Antibody

Cell: HT-29, Concentration: 1:100, Host/Isotype: Rabbit/IgG (blue line), Flow cytometric analysis of Rabbit IgG isotype control on HT-29 (blue) compared with primary antibody (green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Primary Antibody: Rabbit Anti-TLR4 antibody (green line), Isotype Control Antibody: Rabbit IgG (orange line), Secondary Antibody: F (ab')2 fragment goat anti-rabbit IgG-PE (white blue line), Positive control: Jurkat cells (blue line), Protocol, 1.Jurkat cells were washed twice with phosphate-buffered saline (PBS). 2. An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 1 0 min at 37 °C.

Tissue/Cell: rat colon tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PAX8 Polyclonal Antibody, Unconjugated (orb11241) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

U87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PAX8) polyclonal Antibody, Unconjugated (orb11241) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.