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PCNA Antibody
SKU: orb1566579
Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1/1000 IHC: 1/50-1/200 IF: 1/100-1/200 IP: 1/20 |
| Reactivity | Hamster, Human, Monkey, Mouse, Rat |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone No. | 2E1-G10-H10 |
| Immunogen | Purified recombinant human PCNA protein fragments expressed in E.coli. |
| Target | PCNA |
| Molecular Weight | Calculated MW: 29 kDa; Observed MW: 36 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Concentration | 9.03 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Proliferating Cell Nuclear Antigen; DNA polymerase delta auxiliary protein; PCNAR
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Western blot analysis of PCNA in Hela, 3T3, COS7, C6 and CHO-K1 lysates using PCNA antibody.
Immunocytochemistry analysis of PCNA in HeLa cells using PCNA antibody.
Immunoprecipitation analysis of PCNA (2E1) in Hela lysates using PCNA antibody.
Immunohistochemistry analysis of paraffin-embedded Human colorectal carcinoma using PCNA antibody (2E1G10H10).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Quick Database Links
Gene Symbol
PCNA
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
ICC
Immunocytochemistry
IP
Immunoprecipitation
PCNA Antibody (orb1566579)
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