PCNA Rabbit Polyclonal Antibody

SKU: orb1735081

Description

Research Area

Cell Biology

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:2000 - 1:5000, Immunofluorescence 1:500 – 1:1000, Immunohistochemistry (Paraffin) 1:400 – 1:800
Reactivity	Human, Mouse, Rat
Application Notes	Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide
Target	PCNA
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1735081 anti-PCNA antibody (Green) at 1:500 and 4°C.

Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling PCNA with orb1735081 at 1/400.

This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1735081 overnight at 4°C. Lysates/proteins at 35 µg per lane. Anti-PCNA antibody - at 1/3000 dilution.

Quick Database Links

Gene Symbol

PCNA

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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