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PDGF-B Recombinant Protein
SKU: orb1227731
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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human PDGF-BB, His Tag at 5 ug/mL (100 ul/well) can bind Human PDGF R beta, Fc Tag with a linear range of 0.02-1.2 ug/mL. |
| Target | PDGF-B |
| Tag | His Tag & Avi Tag |
| Molecular Weight | 15 kDa |
| Protein Sequence | Ser 82 - Thr 190 |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.085% TFA in 30% ACN |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PDGFBB,PDGFB,FLJ12858,PDGF2,SIS,SSV,c-sis
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Human PDGF-BB Protein, His Tag, Avi Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PDGF-B Recombinant Protein (orb1227731)
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