PDGFRA Antibody (C-term E1063)

SKU: orb1929009

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

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Item 1 of 4

Tested Applications	FC, IHC-P, WB
Dilution range	WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB2005/2006
Target	This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from the C-terminal region of human PDGFRA.
Molecular Weight	122670 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2, CD140a, PDGFRA, PDGFR2, RHEPDGFRA

Quality Guarantee

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Western blot analysis of PDGFRA Antibody (C-term E1063) in K562 cell line lysates (35 ug/lane). PDGFRA (arrow) was detected using the purified Pab.

Western blot analysis of PDGFRA antibody (C-term E1063) in mouse lung tissue lysates (35 ug/lane). PDGFRA (arrow) was detected using the purified Pab.

PDGFRA Antibody (C-term E1063) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from the C-terminal region of human PDGFRA.

UniProt

P16234

RefSeq (Protein):NP_006197.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006197.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Flow Cytometry

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