PDGFRA Antibody (Y720)

SKU: orb1929008

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	IHC-P, WB
Dilution Range	WB - 1:2000, IHC-P - 1:25
Reactivity	Mouse
Predicted Reactivity	Other, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from human PDGFRA. Antigen Region: 698-727 aa.
Target	PDGFRA
Molecular Weight	122670 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2, CD140a, PDGFRA, PDGFR2, RHEPDGFRA

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Anti-PDGFRA Antibody (Y720) at 1:1000 dilution + Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 123 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PDGFRA Antibody (Y720) at 1:2000 dilution + NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 123 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA Antibody (Y720), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Staining PDGFRA in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

PDGFRA

UniProt

P16234

RefSeq (Protein):NP_006197.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006197.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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