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PDIA2 Antibody

SKU: orb1247127

Description

PDIA2 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityMouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Pancreas lysates (calculated MW of 58.2kDa according to NP_006840.2). Recommended concentration: 0.01-0.03µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine and Heart. Recommended concentration: 3.75µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-NLETTKKYAPVD
TargetPDIA2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

PDIA2, PDIP, protein disulfide isomerase family A, member 2 , LA16c-314G4.2, PDA2, PDI, PDIR , Rho GDP dissociation inhibitor gamma, pancreatic protein disulfide isomerase, protein disulfide isomerase, pancreatic, protein disulfide isomerase-associated 2

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PDIA2 Antibody

orb1247127 (0.03 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

PDIA2 Antibody

orb1247127 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

PDIA2 Antibody

orb1247127 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PDIA2 Antibody (orb1247127)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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