CD274 Antibody (Biotin)

SKU: orb1238288

Description

Images & Validation

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Item 1 of 1

Tested Applications	ELISA
Reactivity	Human
Application Notes	PD-L1 antibody can be used for as detection antibody in Sandwich Elisa at 1 μg/mL.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	[8E12]
Immunogen	PD-L1 antibody was raised against the extracellular domain of human PD-L1.
Target	CD274
Purification	PD-L1 Antibody is supplied as protein A purified IgG1.
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PD-L1 Antibody is supplied in PBS containing 1% rAlbumin and 0.02% sodium azide.
Concentration	1 mg/mL
Disclaimer	For research use only

Alternative Names

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PD-L1 Antibody: Programmed cell death 1 ligand-1, programmed death ligand 1, PDL1, PDL-1, B7-H1

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A sandwich ELISA was performed using the anti-PD-L1 mAb orb1239818 (5 μg/ml) as the capture antibody. Biotin-labeled anti-PD-L1 mAb orb1238288 (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 20 pg.

Quick Database Links

Gene Symbol

CD274

UniProt

Q9NZQ7

RefSeq (Protein):NP_054862

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_054862

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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