You have no items in your shopping cart.
CD274 Antibody (Biotin)
SKU: orb751161
Description
Images & Validation
−Item 1 of 9
| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution range | Flow Cytometry (1-2ug/million cells); ,Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | PDL1/2746 |
| Immunogen | Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary) |
| Target | CD274 |
| Source/Expression System | Bioreactor |
| Molecular Weight | 37-50kDa |
| Purification | Protein A |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Buffer/Preservatives | Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. |
| Concentration | Purified Ab conjugated to Biotin |
| Disclaimer | For research use only |
Alternative Names
−Anti-B7 homolog 1 antibody, anti-B7-H1 antibody, anti-CD274 antibody, anti-PD-L1 antibody, anti-PDCD1 ligand 1 antibody, anti-PDCD1L1 antibody, anti-PDCD1LG1 antibody, anti-Programmed cell death 1 ligand 1 antibody
Similar Products
−- Item 1 of 1
Rat Programmed Cell Death Protein 1 Ligand 1 (PDL1) ELISA Kit [orb1736657]
Rat
78.13-5000 pg/mL
30.1 pg/mL
96 T, 48 T - Item 1 of 1
- Item 1 of 2
PD-L1 Rabbit Polyclonal Antibody (Biotin) [orb704075]
WB
Bovine, Equine, Human, Porcine, Rabbit
Mouse, Rat
Rabbit
Polyclonal
Biotin
100 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Analysis of Protein Array containing more than 19000 full-length human proteins using PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Immunofluorescent staining of Jurkat cells. PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot (red).
Flow Cytometric Analysis of human Jurkat cells using PE-conjugated PD-L1 Mouse Monoclonal Antibody (PDL1/2746) (blue); isotype control (red).
Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human breast carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human cervical carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Immunofluorescent staining of Jurkat cells. PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746) (unconjugated). Confirmation of Purity and Integrity of Antibody.
SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746). Confirmation of Purity and Integrity of Antibody.
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD274 Antibody (Biotin) (orb751161)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days