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PEDF rabbit pAb
SKU: orb769440
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PEDF. AA range:258-307 |
| Molecular Weight | 46 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-SERPINF1 antibody, anti-PEDF antibody, anti-PIG35 antibody, anti-Pigment epithelium-derived factor antibody, anti-PEDF antibody, anti-Cell proliferation-inducing gene 35 protein antibody, anti-EPC-1 antibody, anti-Serpin F1 antibody
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Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Immunohistochemistry analysis of PEDF antibody in paraffin-embedded human prostate carcinoma tissue.
Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PEDF rabbit pAb (orb769440)
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