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PER1 Antibody

SKU: orb1255831

Description

PER1 Antibody

Research Area

Epigenetics & Chromatin, Neuroscience

Images & Validation

Tested ApplicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PER1 (NP_002607.2).
TargetPER1
Molecular WeightObserved: 160kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Period circadian protein homolog 1, hPER1, Circadian clock protein PERIOD 1, Circadian pacemaker protein Rigui, PER1, KIAA0482, PER, RIGUI

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PER1 Antibody

Western blot analysis of extracts of various cell lines, using PER1 antibody (orb1255831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

PER1 Antibody

Immunohistochemistry of paraffin-embedded human stomach using PER1 antibody (orb1255831) at dilution of 1:100 (40x lens).

PER1 Antibody

Immunohistochemistry of paraffin-embedded rat kidney using PER1 antibody (orb1255831) at dilution of 1:100 (40x lens).

PER1 Antibody

Immunohistochemistry of paraffin-embedded mouse stomach using PER1 antibody (orb1255831) at dilution of 1:100 (40x lens).

PER1 Antibody

Immunofluorescence analysis of U2OS cells using PER1 antibody (orb1255831) at dilution of 1:100. Blue: DAPI for nuclear staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

PER1 Antibody (orb1255831)

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Available Sizes

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100 μl
$ 830.00
DispatchUsually dispatched within 5-10 working days
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