Peroxidase Conjugated IgG fraction of anti-Goat IgG [H&L] [Mouse]

SKU: orb1674970

Description

Images & Validation

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$Peroxidase Conjugated IgG fraction of anti-Goat IgG [H&L] [Mouse]$

Tested Applications	DOT, ELISA, WB
Reactivity	Goat
Application Notes	This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:6,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Mouse
Immunogen	Goat IgG, whole molecule
Purity	This product is an IgG fraction purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse Serum, Goat IgG and Goat Serum.
Purification	This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse Serum, Goat IgG and Goat Serum.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	10.0 mg/ml (by UV absorbance at 280 nm)
Disclaimer	For research use only

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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