PGC Mouse Monoclonal Antibody

SKU: orb1816836

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Description

Research Area

Amino Acid Metabolism, Antibiotic, Cancer Research, Tumor Biomarkers

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	IHC-P=1:100-500, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b,k
Clone No.	1A4
Immunogen	Recombinant human PGC protein (17-388/388aa)
Target	PGC
Molecular Weight	40 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS (pH7.4) with 0.02% Proclin300.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PEPC_HUMAN; Gastricsin; EC:3.4.23.3; Pepsinogen C; PEPC; PGII; progastricsin;

Quality Guarantee

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Sample: Lane 1: Mouse Stomach tissue lysates, Lane 2: Rat Stomach tissue lysates, Primary: Anti-PGC (orb1816836) at 1/1000 dilution, Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific, Predicted band size: 42 kDa, Observed band size: 39 kDa.

Sample: Lane 1: Recombinant human PGC protein, C-His, Primary: Anti-PGC (orb1816836) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 42 kDa, Observed band size: 40 kDa.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PGC) Monoclonal Antibody, Unconjugated (orb1816836) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PGC

UniProt

P20142

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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