Phospho-AKT (Ser124) Recombinant Rabbit Monoclonal Antibody

SKU: orb559196

Description

Research Area

Akt/PKB Signaling Pathway, Apoptosis, Cell Cycle, Metabolism, Nuclear Signaling Pathway, PI3K/AKT Signaling Pathway

Images & Validation

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Item 1 of 1

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-100, IF=1:50-200
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	2A1
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human Akt1 around the phosphorylation site of Ser124 SG(p-S)PS
Target	AKT1
Molecular Weight	56 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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AKT1(S124); AKT1(phospho Ser124); AKT1(phospho S124); AKT 1; AKT; AKT-1; AKT1_HUMAN; C AKT; cAKT; MGC9965; MGC99656; Oncogene AKT1; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein Kinase B alpha; Protein kinase B; Proto-oncogene c-Akt; RAC alpha; RAC alpha serine/threonine protein kinase; RAC; RAC PK alpha; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1.

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Phospho-AKT (Ser124) Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Lane 3: Human MCF-7 cell lysates, Primary: Anti-phospho-AKT (Ser124) (orb559196) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 56 kDa.