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Phospho-AKT (Ser473) Rabbit Polyclonal Antibody
SKU: orb1294322
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | Western Blot 1:1000 – 1:2000, Immunofluorescence 1:200 – 1:400 |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser473 of human AKT |
| Target | Phospho-AKT (Ser473) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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anti-Phospho-AKT (Ser473) antibody at 1/1000 dilution, Lysates/proteins at 20 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294322 overnight at 4°C.
This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294322 overnight at 4°C. Anti-Phospho-Akt (Ser473) antibody - at 1/1000 dilution Lysates at 30 µg per lane.
Quick Database Links
Gene Symbol
Phospho-AKT (Ser473)
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
Phospho-AKT (Ser473) Rabbit Polyclonal Antibody (orb1294322)
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