Phospho-Akt(Ser473) Antibody

SKU: orb395432

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Description

Mouse monoclonal antibody to Akt (Phospho-Tyr315).

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution range	WB: 1:1000-1:4000; IHC: 1:50-1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Immunogen	v-akt murine thymoma viral oncogene homolog 1
Target	Phospho-Akt(Ser473)
Molecular Weight	60KD
Purity	≥95% as determined by SDS-PAGE
Purification	Protein A+G purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Disclaimer	For research use only

Alternative Names

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AKT, AKT1, AKT1-phospho-S473, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

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Jurkat cells were subjected to SDS PAGE followed by western blot with orb395432 (Phospho-Akt (S473) Antibody) at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb395432 (Phospho-Akt (S473) Antibody) at dilution of 1:200.

Quick Database Links

Gene Symbol

Phospho-Akt(Ser473)

UniProt

P31749

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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