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Phospho-AMPK alpha 1 (Ser496) Recombinant Rabbit Monoclonal Antibody

SKU: orb559194

Description

Phospho-AMPK alpha 1 (Ser496) Recombinant Rabbit Monoclonal Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsICC, WB
Dilution RangeWB=1:500-2000, ICC/IF=1:50-200
ReactivityHuman
Predicted ReactivityMouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.11G1
ImmunogenKLH conjugated Synthesised phosphopeptide derived from mouse AMPK alpha 1 around the phosphorylation site of Ser496 SG(p-S)VS
TargetPRKAA1
Molecular Weight64 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PRKAA1 | AMPK alpha-1 (p-S496); p-AMPK alpha-1; phospho-AMPK alpha-1; AMPKalpha1; C130083N04Rik; AAPK1_MOUSE; Prkaa1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase (ACACA kinase); Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase); Tau-protein kinase PRKAA1; 2.7.11.1; AAPK1_RAT; Ampk1; AMPK; AMPK alpha 1; AMPKa1;
Quality Guarantee

Quality Guarantee

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Phospho-AMPK alpha 1 (Ser496) Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-AMPK alpha 1 (Ser496) (orb559194) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 60 kD.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
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ICC
Immunocytochemistry
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Phospho-AMPK alpha 1 (Ser496) Recombinant Rabbit Monoclonal Antibody (orb559194)

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50 μl
$ 330.00
100 μl
$ 490.00
DispatchUsually dispatched within 5-10 working days
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