Phospho-APG4B (Ser34) Rabbit Polyclonal Antibody

SKU: orb526537

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Description

Research Area

Active Protein-Gene, Autophagy, Cancer Research, Cell Biology, Cell Cycle, Metabolism, Signal Transduction

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Porcine

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human APG4B around the phosphorylation site of Ser34 KY(p-S)IF
Target	ATG4B
Molecular Weight	44 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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APG4B(phospho-Ser34); APG4B(phospho Ser34); APG4B(phospho S34); APG4B(p-Ser34); p-APG4B(Ser34); APG4B; ATG4 autophagy related 4 homolog B(S. cerevisiae); AUT like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353br; ATG4B_HUMAN.

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Phospho-APG4B (Ser34) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-APG4B (Ser34)) Polyclonal Antibody, Unconjugated (orb526537) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Heart tissue lysates, Primary: Anti-phospho-APG4B (Ser34) (orb526537) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

ATG4B

UniProt

Q9Y4P1

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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