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Phospho-c-Jun (Ser63) Recombinant Rabbit Monoclonal Antibody
SKU: orb559120
Featured
Description
Research Area
Cell Signaling, DNA and RNA Research, Intracellular Signaling Proteins, TLR Signaling Pathway, Transcription Factors
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:50-500, IHC-F=1:50-500, ICC/IF=1:50-200, IF=1:50-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 6C2 |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human c-Jun around the phosphorylation site of Ser63 LT(p-S)PD |
| Target | JUN |
| Molecular Weight | 43/36 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−c-Jun(S63); c-Jun(phospho S63); c-Jun(phospho Ser63); p-c-Jun(Ser63); Transcription factor AP-1; Jun oncogene; JUN; AP 1; AP1; AP-1; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN protein; JUNC; p39; Proto oncogene cJun; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog; JUN_HUMAN; Activator 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog.
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-c-Jun (Ser63)) Monoclonal Antibody, Unconjugated (orb559120) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-c-Jun (Ser63)) Monoclonal Antibody, Unconjugated (orb559120) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-c-Jun (Ser63)) Monoclonal Antibody, Unconjugated (orb559120) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 4: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-c-Jun (Ser63) (orb559120) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36/43 kD, Observed band size: 43 kD.
Quick Database Links
Gene Symbol
JUN
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
Phospho-c-Jun (Ser63) Recombinant Rabbit Monoclonal Antibody (orb559120)
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