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Phospho-CRMP2 (Thr514) Rabbit Polyclonal Antibody

SKU: orb5917
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Description

Phospho-CRMP2 (Thr514) Rabbit Polyclonal Antibody

Research Area

Alzheimer's Disease, Axonal Guidance Proteins, Neurogenesis, Neuroscience

Images & Validation

Tested ApplicationsIF, IHC-P, WB
Dilution RangeWB=1:500-2000, IHC-P=1:100-500, IF=1:100-500
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Equine, Gallus, Rabbit

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human CRMP-2 around the phosphorylation site of Thr514 TV(p-T)PA
TargetDPYSL2
Molecular Weight62 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CRMP2(phospho T514); p-CRMP2(phospho T514); CRMP2(phospho T514); p-CRMP2(phospho T514); CRMP2(phospho T514); CRMP-2(phospho Thr514); p-CRMP2(Thr514); Collapsin response mediator protein 2; Collapsin response mediator protein hCRMP 2; CRAM; CRMP 2; CRMP-2; CRMP2; DHPRP 2; DHPRP2; Dihydr pyrimidinase 2; Dihydropyrimidinase 2; Dihydropyrimidinase like 2; Dihydropyrimidinase like 2 long form; Dihydropyrimidinase related protein 2; Dihydropyrimidinase-related protein 2; DPYL 2; DPYL2; DPYL3_HUMAN; DPYSL 2; Dpysl2; DRP 2; DRP-2; DRP2; Musunc 33; Musunc33; N2A3; TOAD 64; TOAD64; ULIP 2 protein; ULIP-2; Ulip2; Unc-33-like phosphoprotein 2.

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Phospho-CRMP2 (Thr514) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-CRMP2 (Thr514)) Polyclonal Antibody, Unconjugated (orb5917) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Phospho-CRMP2 (Thr514) Rabbit Polyclonal Antibody

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Phospho-CRMP2 (Thr514) (orb5917) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 65 kD.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol

Phospho-CRMP2 (Thr514) Rabbit Polyclonal Antibody (orb5917)

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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