Phospho-E2F1 (Ser337) Rabbit Polyclonal Antibody

SKU: orb156678

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Description

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human E2F1 around the phosphorylation site of Ser337 L(p-S)PL
Target	E2F1
Molecular Weight	46 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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phospho-E2F1(Ser337); p-E2F1(phospho-Ser337); E2F1(phospho S337); E2F 1; E2F transcription factor 1; E2F-1; E2f1 E2F transcription factor 1; KIAA4009; mKIAA4009; OTTHUMP00000030661; PBR 3; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP 3; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma associated protein 1; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1; E2F1_HUMAN.

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Phospho-E2F1 (Ser337) Rabbit Polyclonal Antibody

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-E2F1 (Ser337) (orb156678) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55-60 kD, Observed band size: 55-60 kD.

Sample: Lane 1: Siha (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-E2F1 (Ser337) (orb156678) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55-60 kD, Observed band size: 55-60 kD.

Quick Database Links

Gene Symbol

E2F1

UniProt

Q01094

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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