Phospho-Eph receptor B1 (Tyr928) Rabbit Polyclonal Antibody

SKU: orb156749

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Description

Research Area

Axonal Guidance Proteins, Cell Biology, Eph Eeceptors, Kinases, Neuroscience, Receptor Tyrosne Kinases, Tyrosine Kinase Signaling Pathway

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr928 VQ(p-Y)RD
Target	EPHB1
Molecular Weight	106 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Eph receptor B1(phospho Y928); EphB1(phospho Y928); Cek 6; Elkh; Eph tyrosine kinase 2; EPH2; EphB1; Ephrin receptor Eph B1 precursor; Ephrin type B receptor 1; HEK 6; NET; Neuronally expressed EPH related tyrosine kinase; Tyrosine protein kinase receptor EPH 2; ELK; EPH-like kinase 6; Ephb1; EPHB1_HUMAN; Ephrin type B receptor 1; Ephrin type-B receptor 1; EPHT2; HEK6; Neuronally-expressed EPH-related tyrosine kinase; Tyrosine protein kinase receptor EPH 2; Tyrosine-protein kinase receptor EPH-2.

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Phospho-Eph receptor B1 (Tyr928) Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse NIH/3T3 cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human U87MG cell lysates, Lane 5: Human MCF-7 cell lysates, Lane 6: Human U-2 OS cell lysates, Primary: Anti-phospho-Eph receptor B1 (Tyr928) (orb156749) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 106 kDa, Observed band size: 130 kDa.

Sample: Ovary (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Eph receptor B1 (Tyr928) (orb156749) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 106 kD, Observed band size: 121 kD.

Tissue/Cell: Rat brain, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-EphB1/Eph receptor B1 (Tyr928) Polyclonal Antibody, Unconjugated (orb156749) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

EPHB1

UniProt

P54762

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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