You have no items in your shopping cart.
Phospho-ERBB2(Y1127) Antibody
SKU: orb1930996
Description
Research Area
Cancer Biology, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | DOT, IF, WB |
|---|---|
| Dilution range | WB - 1:5000, IF - 1:100, DB - 1:500 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 137910 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−HER2, MLN19, NEU, NGL
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Dot blot analysis of Phospho-ERBB2-Y1127 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Fluorescent confocal image of MCF7 cells stained with phospho-ERBB2-Y1127 antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with phospho-ERBB2- Y1127 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using Phospho-ERBB2-Y1127 or ERBB2 antibody (right).
Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using phospho ERBB2-Y1127 (left) or ERBB2 antibody (right)
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
DOT
Dot Blot
Phospho-ERBB2(Y1127) Antibody (orb1930996)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days